Analysis Note

ControlBreast Cancer, Colon, Skin tissue

Application

Research Sub CategoryTranscription Factors

Western Blot (on recombinant protein only)

Research CategoryEpigenetics & Nuclear Function

Detect acetyl-p53 (Lys373) with Anti-acetyl-p53 Antibody (Lys373, Lys382) (Rabbit Polyclonal Antibody), that has been shown to work in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

p53 is a DNA-binding, oligomerization domain and transcription activation domain-containing tumor suppressor that upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 polyubiquitinates p53 for proteasome targeting. p53 can assemble into tetramers in the absence of DNA, fluctuates between latent and active (DNA-binding) conformations, and is differentially activated through posttranslational modifications including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) (amino acids 110-286) of p53 can compromise energetically favorable association with cis elements and are implicated in several human cancers.

Immunogen

peptide of human p53 corresponding to amino acids 368-389 with a duplication of amino acids 376 and 377 (HLKSKACKGQSTSTSRHKACKLMFKTC) where ACK denotes acetyl-lysine

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1137

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Rabbit polyclonal antiserum containing 0.05% sodium azide. Frozen solution.

Quality

routinely evaluated by an in vitro acetyl transferase assay on recombinant p300, HAT domain (Catalog #14-418)

Specificity

Recognizes p53 acetylated in vitro by recombinant p300, and for peptide containing acetyl-lysine 373 but not for the peptide containing acetyl-lysine 320.

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

53 kDa